Recombinant Protein G, His-tag

Species

Gene ID

Accession

Source

Length

MW

Tag

－

－

－

E. coli

210aa

29kDa

His

About this Protein

Name

Recombinant Protein G, His-tag (Recombinant Protein G, His-tag; Protein G, His-tag)；重组蛋白G，His标签

Synonyms

N/A

Purity

>97% by SDS-PAGE and RP-HPLC analyses

Biological Activity

The recombinant Protein G is a genetically engineered protein containing 3 IgG-binding regions of protein G. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein G to ensure the maximum specific IgG binding. The recombinant Protein G is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein G binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG3, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2a, IgG2c). It also binds to total IgG from cow, goat, sheep, house and rabbit.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from additive free solution.

Endotoxin

Less than 0.1EU/μg of Protein G, His-tag as determined by LAL method.

Reconstitution

Dissolve in distilled water or saline.

Category

Protein G

Background

Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.

Amino Acid Sequence

MGTYKLILNG KTLKGETTTE AVDAATAEKV FKQYANDNGV DGEWTYDDAT KTFTVTEKPE VIDASELTPA VTTYKLVING KTLKGETTTE AVDAATAEKV FKQYANDNGV DGEWTYDDAT KTFTVTEKPE VIDASELTPA VTTYKLVING KTLKGETTTK AVDAETAEKA FKQYANDNGV DGVWTYDDAT KTFTVTEKLA AALEHHHHHH

产品编号

产品名称

包装

P5030-1mg

Recombinant Protein G, His-tag

1mg

P5030-10mg

Recombinant Protein G, His-tag

10mg

P5030-1g

Recombinant Protein G, His-tag

1g

-

说明书

1份