Recombinant Rat bFGF

Species

Gene ID

Accession

Source

Length

MW

Tag

Rat

54250

P13109

E. coli

146aa

16.4kDa

－

About this Antibody

Name

Recombinant Rat bFGF (Recombinant Rat Basic Fibroblast Growth Factor; rRtbFGF)；重组大鼠碱性成纤维细胞生长因子

Synonyms

FGF2; FGF-2; FGF2AS; GFG1; HBGH-2; NUDT6; Prostatropin; Basic fibroblast growth factor; basic fibroblast growth factor bFGF; bFGF; BFGF; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.2ng/ml, corresponding to a specific activity of >5.0×10⁶IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rRtbFGF as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Rat bFGF, encoded by the FGF2 gene, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named "acidic fibroblast growth factor" (FGF-1) and "basic fibroblast growth factor" (FGF-2). Rat bFGF shares 96% amino acid sequence identity with human bFGF, and 98% amino acid sequence identity with mouse. Affinity between bFGF and its receptors can be increased by heparin or heparan sulfate proteoglycan. bFGF plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. bFGF are also involved in a variety of biological processes, including embryonic development , morphogenesis, tissue repair, tumor growth and invasion. Additionally, bFGF is frequently used for a critical component of cell culture medium, e.g., human embryonic stem cell culture medium, serum-free culture systems.

Amino Acid Sequence

MPALPEDGGG AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS

产品编号

产品名称

包装

P6348-10μg

Recombinant Rat bFGF

10μg

P6348-50μg

Recombinant Rat bFGF

50μg

P6348-100μg

Recombinant Rat bFGF

100μg

P6348-1mg

Recombinant Rat bFGF

1mg

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说明书

1份