Recombinant Rat CT-1

Species

Gene ID

Accession

Source

Length

MW

Tag

Rat

29201

Q63086

E. coli

203aa

21.4kDa

－

About this Antibody

Name

Recombinant Rat CT-1 (Recombinant Rat Cardiotrophin-1; rRtCT-1)；重组大鼠心肌营养素1

Synonyms

CTF1; cardiophin 1; cardiotrophin 1; cardiotrophin-1; CT-1CT1

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5ng/ml, corresponding to a specific activity of >2.0×10⁶IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 30% Acetonitrile, 0.1% TFA.

Endotoxin

Less than 1EU/μg of rRtCT-1 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4mM HCl to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Others

Background

Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). CT-1 is a pleiotropic cytokine which is expressed in various tissues including the adult heart, skeletal muscle, ovary, colon, prostate and fetal lung. Studies showed CT-1 which induces cardiac myocyte hypertrophy in vitro can bind to and activate the ILST/gp130 receptor. Rat CT-1 encodes a 203 amino acid (a.a.) residue protein that lacks a hydrophobic signal peptide and it shares 94%a.a. and 79%a.a. sequence identity with human and murine CT-1.

Amino Acid Sequence

MSQREGSLED HQTDSSFSFL PHLEAKIRQT HNLARLLTKY ADQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSALPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP VPEPVATSAL FTSNSAAGVF SAKVLGLHVC GLYGEWVSRT EGDLGQLVPG GVA

产品编号

产品名称

包装

P7370-2μg

Recombinant Rat CT-1

2μg

P7370-10μg

Recombinant Rat CT-1

10μg

P7370-100μg

Recombinant Rat CT-1

100μg

P7370-1mg

Recombinant Rat CT-1

1mg

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说明书

1份