Recombinant Rat EGF

Species

Gene ID

Accession

Source

Length

MW

Tag

Rat

25313

P07522

E. coli

53aa

6.1kDa

－

About this Protein

Name

Recombinant Rat EGF (Recombinant Rat Epidermal Growth Factor; rRtEGF)；重组大鼠表皮生长因子

Synonyms

HOMG4; URG; Urogastrone; beta-urogastrone; epidermal growth factor; epidermal growth factor (beta-urogastrone); HOMG4; pro-epidermal growth factor; URG

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1ng/ml, corresponding to a specific activity of >1.0×10⁷IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rRtEGF as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Epidermal Growth Factor (EGF) was originally discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition, and stunting of growth when injected into newborn mice. It is prototypic of a family of growth factors that are derived from membrane-anchored precursors. All members of this family are characterized by the presence of at least one EGF structural unit (defined by the presence of a conserved 6 cysteine motif that forms three disulfide bonds) in their extracellular domain. EGF is initially synthesized as a 130kDa precursor transmembrane protein containing 9 EGF units. The mature soluble EGF sequence corresponds to the EGF unit located proximal to the transmembrane domain. The membrane EGF precursor is capable of binding to the EGF receptor and was reported to be biologically active. Mature rat EGF shares 70%a.a. sequence identity with mature human EGF.

Amino Acid Sequence

NSNTGCPPSY DGYCLNGGVC MYVESVDRYV CNCVIGYIGE RCQHRDLRWW KLR

产品编号

产品名称

包装

P6375-20μg

Recombinant Rat EGF

20μg

P6375-100μg

Recombinant Rat EGF

100μg

P6375-1mg

Recombinant Rat EGF

1mg

-

说明书

1份