Recombinant Rat FGF-21

Species

Gene ID

Accession

Source

Length

MW

Tag

Rat

170580

Q8VI80

E. coli

180aa

19.7kDa

－

About this Antibody

Name

Recombinant Rat FGF-21 (Recombinant Rat Fibroblast Growth Factor-21; rRtFGF-21)；重组大鼠成纤维细胞生长因子21

Synonyms

FGF-21; fibroblast growth factor 21

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5μg/ml, corresponding to a specific activity of >2.0×10³IU/mg in the presence of 5μg/ml of rMuKlotho-β and 10μg/ml of heparin.

Physical Appearance

White lyophilized powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, 500mM NaCl, pH7.4.

Endotoxin

Less than 1EU/μg of rRtFGF-21 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Fibroblast growth factor 21 (FGF-21) encoded by the FGF-21 gene belongs to the large FGF family and it is specifically induced by HMGCS2 activity. In mice, brown adipose tissue becomes a source of systemic FGF-21 after cold exposure. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. Recombinant rat FGF-21 contains 180 amino acids residues and show limited binding to heparin. In addition, rat FGF-21 respectively shows 81% and 92%a.a. identity to human and murine FGF-21, and it show activity on murine and human cells.

Amino Acid Sequence

AYPISDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGTAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GTLYGSPHFD PEACSFRELL LKDGYNVYQS EAHGLPLRLP QKDSQDPATR GPVRFLPMPG LPHEPQEQPG VLPPEPPDVG SSDPLSMVEP LQGRSPSYAS

产品编号

产品名称

包装

P6366-5μg

Recombinant Rat FGF-21

5μg

P6366-25μg

Recombinant Rat FGF-21

25μg

P6366-100μg

Recombinant Rat FGF-21

100μg

P6366-1mg

Recombinant Rat FGF-21

1mg

-

说明书

1份