Recombinant Rat IL-1RA

Species

Gene ID

Accession

Source

Length

MW

Tag

Rat

60582

P25086

E. coli

152aa

17.4kDa

－

About this Protein

Name

Recombinant Rat IL-1RA (Recombinant Rat Interleukin-1 Receptor Antagonist; rRtIL-1RA)；重组大鼠白细胞介素1受体拮抗剂

Synonyms

IL-1F3; IL1RN; DIRA; ICIL-1RA; IL1F3; IL1RA; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 150ng/ml, corresponding to a specific activity of >6.7×10³IU/mg in the presence of 50pg/ml rRtIL-1α.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rRtIL-1RA as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. IL-1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. The rat IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Rat IL-1RA shares 89% and 73%a.a. sequence homology with murine and human IL-1RA.

Amino Acid Sequence

HPAGKRPCKM QAFRIWDTNQ KTFYLRNNQL IAGYLQGPNT KLEEKIDMVP IDFRNVFLGI HGGKLCLSCV KSGDDTKLQL EEVNITDLNK NKEEDKRFTF IRSETGPTTS FESLACPGWF LCTTLEADHP VSLTNTPKEP CTVTKFYFQE DQ

产品编号

产品名称

包装

P6249-20μg

Recombinant Rat IL-1RA

20μg

P6249-100μg

Recombinant Rat IL-1RA

100μg

P6249-1mg

Recombinant Rat IL-1RA

1mg

-

说明书

1份