Recombinant Rat IL-1β

Species

Gene ID

Accession

Source

Length

MW

Tag

Rat

24494

Q5BKB0

E. coli

153aa

17.3kDa

－

About this Antibody

Name

Recombinant Rat IL-1β (Recombinant Rat Interleukin-1β; rRtIL-1β)；重组大鼠白细胞介素1β

Synonyms

IL1B; IL-1F2; catabolin; Catabolin; IL-1; IL-1B; IL1-BETA; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.1ng/ml, corresponding to a specific activity of >1.0×10⁷IU/mg.

Physical Appearance

Sterile Filtered lyophilized (freeze-dried) liquid-like powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, with 5% trehalose, 0.02% Tween-20.

Endotoxin

Less than 1EU/μg of rRtIL-1β as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-1beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1αand IL-1β binds to the same receptor and has similar but not identical biological properties. The mature rat IL-1β shares 90%a.a. sequence identity with cotton rat and mouse and 65% to 77% with canine, human and rhesus IL-1β.

Amino Acid Sequence

MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSS

产品编号

产品名称

包装

P6245-2μg

Recombinant Rat IL-1β

2μg

P6245-10μg

Recombinant Rat IL-1β

10μg

P6245-100μg

Recombinant Rat IL-1β

100μg

P6245-1mg

Recombinant Rat IL-1β

1mg

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说明书

1份