Recombinant Rat IL-21

Species

Gene ID

Accession

Source

Length

MW

Tag

Rat

365769

A3QPB9

E. coli

129aa

15.2kDa

－

About this Antibody

Name

Recombinant Rat IL-21 (Recombinant Rat Interleukin-21; rRtIL-21)；重组大鼠白细胞介素21

Synonyms

IL-21Za11interleukin-21; interleukin 21; interleukin-21 isoform

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human N1186 cells is less than 50ng/ml, corresponding to a specific activity of >2.0×10⁴IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rRtIL-21 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Rat IL-21 is produced by CD4⁺ T cells in response to antigenic stimulation and can regulating immune system cells, for instance cytotoxic T cells and natural killer cells. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation with IL-4 of IgG production, and induction of apoptotic effects in naïve B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, it promotes the anti-tumor activity of CD8⁺ T-cells and NK cells. IL-21 elicits its effect through binding to IL-21R, which also contains the gamma chain found in other cytokine receptors such as IL-2, IL-4, IL-7, IL-9 and IL-15. IL-21 shows having much relation with clinical illnesses, including cancer immunotherapy, viral infections and allergies. Mature rat IL-21 shares 88%a.a. sequence identity with mouse IL-21.

Amino Acid Sequence

HKSSPQRPDH LLIRLRHLMD IVEQLKIYEN DLDPELLTAP QDVKGQCEHE AFACFQKAKL KPSNTGNNKT FINDLLAQLR RRLPAKRTGN KQRHMAKCPS CDLYEKKTPK EFLERLKWLL QKMIHQHLS

产品编号

产品名称

包装

P6308-2μg

Recombinant Rat IL-21

2μg

P6308-10μg

Recombinant Rat IL-21

10μg

P6308-100μg

Recombinant Rat IL-21

100μg

P6308-1mg

Recombinant Rat IL-21

1mg

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说明书

1份