Recombinant Rat MIP-1β/CCL4

Species

Gene ID

Accession

Source

Length

MW

Tag

Rat

116637

P50230

E. coli

69aa

7.8kDa

－

About this Antibody

Name

Recombinant Rat MIP-1β/CCL4 (Recombinant Rat Macrophage Inflammatory Protein-1β/CCL4; rRtMIP-1β/CCL4)；重组大鼠巨噬细胞炎症蛋白1β/CCL4

Synonyms

Exodus-3; MIP-1 beta; ACT2; ACT-2; AT744.1; C-C motif chemokine 4; chemokine (C-C motif) ligand 4; G-26; G-26 T-lymphocyte-secreted protein; HC21; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta(1-69); PAT 744; Protein H400; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-1000ng/ml.

Physical Appearance

Sterile Filtered lyophilized (freeze-dried) liquid-like powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, pH7.4, 3% trehalose.

Endotoxin

Less than 1EU/μg of rRtMIP-1β/CCL4 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Chemokines

Background

Chemokine (C-C motif) ligand 4 encoded by the CCL4 gene, also known as macrophage inflammatory protein-1β (MIP-1β) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8⁺ T cells. In addition, it is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Furthermore, recombinant rat CCL4 contains 69 amino acids and it shares 80% and 86%a.a. sequence identity with human and murine CCL4. Both human and murine MIP-1α and MIP-1β are active on human and murine hematopoietic cells.

Amino Acid Sequence

APIGSDPPTS CCFSYTSRKI HRNFVMDYYE TSSLCSQPAV VFLTKKGRQI CADPSEPWVN EYVNDLELN

产品编号

产品名称

包装

P6911-5μg

Recombinant Rat MIP-1β/CCL4

5μg

P6911-20μg

Recombinant Rat MIP-1β/CCL4

20μg

P6911-100μg

Recombinant Rat MIP-1β/CCL4

100μg

P6911-1mg

Recombinant Rat MIP-1β/CCL4

1mg

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说明书

1份