Recombinant Rat PDGF-AA

Species

Gene ID

Accession

Source

Length

MW

Tag

Rat

25266

P28576

E. coli

111aa

25.3kDa

－

About this Antibody

Name

Recombinant Rat PDGF-AA (Recombinant Rat Platelet-derived Growth Factor-AA; rRtPDGF-AA)；重组大鼠血小板衍生生长因子-AA

Synonyms

Platelet-derived growth factor subunit A; PDGF subunit A; PDGF-1; Platelet-derived growth factor A chain; Platelet-derived growth factor alpha polypeptide; Pdgfa; Rpa1

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 5.0ng/ml, corresponding to a specific activity of >2.0×10⁵IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 30% Acetonitrile and 0.1% TFA.

Endotoxin

Less than 0.1EU/μg of rRtPDGF-AA as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4mM HCl to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in animal study by Lynch and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three subforms: PDGF-AA, PDGF-BB, PDGF-AB. It is involved in a number of biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is mediated by two receptors (PDGFR-α and PDGFR-β).

Amino Acid Sequence

MSIEEAIPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETDV R

产品编号

产品名称

包装

P6393-2μg

Recombinant Rat PDGF-AA

2μg

P6393-10μg

Recombinant Rat PDGF-AA

10μg

P6393-100μg

Recombinant Rat PDGF-AA

100μg

P6393-1mg

Recombinant Rat PDGF-AA

1mg

-

说明书

1份