Recombinant Rhesus Macaque Flt-3 Ligand

Species

Gene ID

Accession

Source

Length

MW

Tag

Rhesus Macaque

719239

H9Z6V7

E. coli

159aa

18kDa

－

About this Antibody

Name

Recombinant Rhesus Macaque Flt-3 Ligand (Recombinant Rhesus Macaque Fms-related Tyrosine Kinase 3 Ligand; rRhFlt-3L)；重组恒河猴Fms相关酪氨酸激酶3配体

Synonyms

Fms-related tyrosine kinase 3 ligand; FLT3LG

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human Aml5 cells is less than 1.0ng/ml, corresponding to a specific activity of >1.0×10⁶IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rRhFlt-3L as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Flt-3 ligand (FL) is a recently identified hematopoietic cytokine whose activities are mediated by binding to the transmembrane glycoprotein Flt-3. Flt-3 was first discovered as a member of the class III subfamily of receptor tyrosine kinases (RTK) whose expression among hematopoietic cells was found to be restricted to highly enriched stem/progenitor cell populations. Additionally, class III RTKs include the receptors from SCF, M-CSF and PDGF. Not surprisingly, Flt-3 ligand is also structurally related to M-CSF and SCF. All three cytokines have been shown to exist both as type I transmembrane proteins and as soluble proteins. The predominant human FL isoform is a transmembrane protein that can undergo proteolytic cleavage to generate a soluble form of the protein. FL has been shown to synergize with a wide variety of hematopoietic cytokines to stimulate the growth and differentiation of early hematopoietic progenitors.

Amino Acid Sequence

TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVPSNLQD EELCGALWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQHP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPRSPGALEA TALTAPQRP

产品编号

产品名称

包装

P5885-2μg

Recombinant Rhesus Macaque Flt-3 Ligand

2μg

P5885-10μg

Recombinant Rhesus Macaque Flt-3 Ligand

10μg

P5885-100μg

Recombinant Rhesus Macaque Flt-3 Ligand

100μg

P5885-1mg

Recombinant Rhesus Macaque Flt-3 Ligand

1mg

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说明书

1份