Recombinant Rhesus Macaque IL-3

Species

Gene ID

Accession

Source

Length

MW

Tag

Rhesus Macaque

706946

P25140

E. coli

124aa

14kDa

－

About this Antibody

Name

Recombinant Rhesus Macaque IL-3 (Recombinant Rhesus Macaque Interleukin-3; rRhIL-3)；重组恒河猴白细胞介素3

Synonyms

Interleukin-3; IL-3; Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor; IL3

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Assay #1: Fully biologically active when compared to standard. The ED50 as determined by the dosedependent stimulation of the proliferation of murine NFS-60 cells is less than 5.0ng/ml, corresponding to a specific activity of >2×10⁵IU/mg. Assay #2: The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.05ng/ml, corresponding to a specific activity of >2.0×10⁷IU/mg.

Physical Appearance

Sterile Filtered lyophilized (freeze-dried) liquid-like powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, 5% trehalose.

Endotoxin

Less than 1EU/μg of rRhIL-3 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-3 (IL-3) is an interleukin, a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. The rhesus macaque IL-3 reported to be a monomer, as it is known, contains 124 amino acids residues which is a single non-glycosylated polypeptide. Specifically, recombinant rhesus macaque, human and murine IL-3 share low homology.

Amino Acid Sequence

APMTQTTSLK TSWAKCSNMI DEIITHLNQP PLPSPDFNNL NEEDQTILVE KNLRRSNLEA FSKAVKSLQN ASAIESILKN LPPCLPMATA APTRPPIRIT NGDRNDFRRK LKFYLKTLEN EQAQ

产品编号

产品名称

包装

P5844-2μg

Recombinant Rhesus Macaque IL-3

2μg

P5844-10μg

Recombinant Rhesus Macaque IL-3

10μg

P5844-100μg

Recombinant Rhesus Macaque IL-3

100μg

P5844-1mg

Recombinant Rhesus Macaque IL-3

1mg

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说明书

1份