Recombinant Rhesus Macaque TNF-α/TNFSF2

Species

Gene ID

Accession

Source

Length

MW

Tag

Rhesus Macaque

715467

P48094

E. coli

157aa

17.3kDa

－

About this Antibody

Name

Recombinant Rhesus Macaque TNF-α/TNFSF2 (Recombinant Rhesus Macaque Tumor Necrosis Factor-α; rRhTNF-α)；重组恒河猴肿瘤坏死因子α

Synonyms

Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Tumor necrosis factor, membrane form; N-terminal fragment; NTF; Intracellular domain 1; ICD1; Intracellular domain 2; ICD2; C-domain 1; C-domain 2; Tumor necrosis factor, soluble form; TNF; TNFA; TNFSF2

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05ng/ml, corresponding to a specific activity of >2.0×10⁷IU/mg in the presence of actinomycin D.

Physical Appearance

Sterile Filtered lyophilized (freeze-dried) liquid-like powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, 5% trehalose.

Endotoxin

Less than 1EU/μg of rRhTNF-α as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Rhesus macaque and human TNF-α show approximately 98% homology at the amino acid level. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.

Amino Acid Sequence

VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELTDNQLVV PSEGLYLIYS QVLFKGQGCP SNHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPD YLDFAESGQV YFGIIAL

产品编号

产品名称

包装

P5880-5μg

Recombinant Rhesus Macaque TNF-α/TNFSF2

5μg

P5880-25μg

Recombinant Rhesus Macaque TNF-α/TNFSF2

25μg

P5880-100μg

Recombinant Rhesus Macaque TNF-α/TNFSF2

100μg

P5880-1mg

Recombinant Rhesus Macaque TNF-α/TNFSF2

1mg

-

说明书

1份