CAPN3 protein

Protein Name

Human CAPN3 protein

Protein Symbol

CAPN3

Original Source

Expression System

GenBank Accession #

BC003521.1

Uniprot Accession #

Q9BQC8

Description

Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. Calpains are a group of calcium-sensitive cysteine proteases that are ubiquitously expressed in mammals. This family contains 14 members with mu-calpain (calpain 1) and m-calpain (calpain 2) being the most well-characterized. Structurally, calpains contain two subunits; an 80 kDa catalytic subunit and a 28 kDa regulatory subunit that functions as a chaperone to stabilize the 80 kDa structure. Calpains are regulated by Ca2+ concentration, phosphorylation, calpastatin and probably by altering their subcellular localization(limiting access to substrate). These endopeptidases have numerous functions including, but not limited to, remodeling of cytoskeletal attachments to the plasma membrane during cell fusion and cell motility, proteolytic modification of molecules in signal transduction pathways, degradation of enzymes controllingprogression through the cell cycle, regulation of gene expression, substrate degradation in some apoptoticpathways, and an involvement in long-term potentiation. Perturbations in calpain activity have beenassociated in pathophysiological processes contributing to type II diabetes (calpain 10), Alzheimer's disease (calpain 1), gastric cancer (calpain 9) and muscular dystrophy (calpain 3).

Application

WB, ELISA, IP, antibody production, protein array

Fusion tag

N-His

Peptide Length

171aa(including fusion tag)

Molecular Weight

20.2kDa(including fusion tag)

pI

7.0

Activity

NA

Storage

Storage buffer: 20mM Tris.Cl, 50mM NaCl, 20% Glycerol, pH9.0. Store at -80°C and avoid repeated freeze-thaw cycles.