Bolt Bis-Tris Plus Gels

Bolt Bis-Tris Plus Gels are precast polyacrylamide gels developed to provide optimal separation of a wide range of protein sizes under denaturing conditions. Bolt Bis-Tris Plus Gels are designed to deliver consistent gel performance with a neutral pH environment that minimizes protein modifications. As such, Bolt gels are a good choice before downstream transfer and western blot analysis and any other technique where protein integrity is crucial.

Features of Bolt Bis-Tris Plus Gels include:

Better protein integrity— preserves your proteins with neutral pH sample preparation process
Optimized run conditions—separates your proteins in approximately 35 minutes using constant voltage
Wedge-shaped wells—helps you achieve easier sample loading and up to two times higher sample volume
Long shelf life—allows you to store gels for at least 16 months at 4–25°C

Bolt Bis-Tris Plus Gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use buffers containing SDS. The gels can be run using MES SDS running buffer to better resolve smaller proteins (0.3–260 kDa) and MOPS SDS running buffer to resolve medium- to large-size proteins (14–260 kDa).