SMER28 (Autophagy增强剂)

化学名

6-bromo-N-prop-2-enylquinazolin-4-amine

简称

SMER28

别名

AC1LTGZ0, SMER26

中文名

N/A

化学式

C₁₁H₁₀BrN₃

分子量

264.12

CAS号

307538-42-7

纯度

98%

溶剂/溶解度

Water<1mg/ml; DMSO52mg/ml; Ethanol52mg/ml

溶液配制

5mg加入1.89ml DMSO，或者每2.64mg加入1ml DMSO，配制成10mM溶液。SC5502-10mM用DMSO配制。

产品描述

SMER28 is a small-molecule enhancer (SMER) of autophagy, inducing autophagy independently of rapamycin in mammalian cells.

信号通路

Autophagy

靶点

Autophagy

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IC50

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体外研究

SMER28 is a positive regulator of autophagy in a mechanism independent from the mTOR pathway. It increases autophagosome synthesis and enhances the clearance of model autophagy substrates such as A53T a-synuclein and mutant huntingtin fragments. A marked decrease in Aβ/APP-CTF levels and enhancement for degradation of Aβ/APP-CTF via autophagy is also induced by SMER28. APP-CTF and LC3-II are cocompartmentalized on SMER28 treatment.

体内研究

N/A

临床实验

N/A

特征

N/A

酶活性检测实验

方法

N/A

细胞实验

细胞系

MEF cells overexpressing βCTF/ N2a-APP cells/ Rat primary neuronal cultures

浓度

10μM in MEF cells/0-50μM in N2a-APP cells and rat primary neuronal cultures

处理时间

16hrs

方法

MEF cells overexpressing βCTF are treated with autophagy-enhancing compounds (10μM) for 16 h. Cell lysates are analyzed by SDS-PAGE and Western blotting using anti-βCTF (6E10) and anti-tubulin antibodies.

动物实验

动物模型

N/A

配制

N/A

剂量

N/A

给药方式

N/A

产品编号

产品名称

包装

SC5502-10mM

SMER28 (Autophagy增强剂)

10mM×0.2ml

SC5502-5mg

SMER28 (Autophagy增强剂)

5mg

SC5502-25mg

SMER28 (Autophagy增强剂)

25mg

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说明书

1份