HDAC7 兔单克隆抗体

背景资料

Human HDAC7 is composed of 912 amino acid residues. Although HDAC7 is localized mostly to the cell nucleus， it is also found in the cytoplasm， suggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a carboxy-terminal domain and is dependent on interaction with class I HDACs in the nucleus. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions， leading to the inhibition of gene transcription.

应用类型

Western blotting （0.5-4 ug/ml）， immunoprecipitation （20-40 ug/ml） and Immunohistochemistry （20-40 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects ~105 kDa HDAC7. Mouse small intestine tissue lysate can be use as a positive control. The antibody does not recognize other members of the HDAC family.

免疫原

Synthetic peptide surrounding amino acid 921 of murine HDAC7

来源宿主

兔

反应性

经检测验证，可与人、小鼠、大鼠种属来源的HDAC7蛋白发生免疫反应.

保存建议

提供的HDAC7抗体产品为100 ug 无色溶液，其浓度为0.2 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该HDAC7抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.