SIRT1 兔单克隆抗体

背景资料

The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases （class III HDACs） and have been shown to catalyze a reaction that couples lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose from NAD+ and the abstracted acetyl group. This SIRT family of genes is highly conserved from prokaryotes to eukaryotes and is involved in diverse processes including transcriptional regulation， cell cycle progression， DNA damage repair and aging. SIRT1 has been shown to be induced by caloric restriction （CR） in several tissues and SIRT1 mediated deacetylase activity attenuates B-amyloid content in the brain during CR. SIRT1 also regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53.

应用类型

Western blot: 1:500  1:2000 dilution， IHC: 1:50 - 1:200 dilution. However， the optimal conditions should be determined individually.

免疫原

C terminal peptide of human SIRT1

来源宿主

兔

反应性

经检测验证，可与人、小鼠、大鼠种属来源的SIRT1蛋白发生免疫反应.

保存建议

提供的SIRT1抗体产品为无色溶液形式，含有100 ug抗体.该产品浓度为与批次相关，溶解于包含有50% 甘油和0.02%叠氮化钠的PBS缓冲液（pH 7.3）中.请在收到该SIRT1抗体产品后，将其置于-20 °C冷冻保存，建议分装使用以避免反复冻融.