P53抗体(小鼠单抗)

背景资料

p53， a 53 kDa protein， binds to a DNA consensus sequence， the p53 response element， and regulates normal cell cycle events by activating transcription of genes involved either in progression through the cell cycle， or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the concentration in normal cells， principally due to the increased half-life （4 hrs） compared to that of wild-type （20 min）. p53 localizes in the nucleus， but is detectable at the plasma membrane during mitosis and certain mutations also modulate cytoplasmic/nuclear distribution. p53 downregulates Bcl-2 expression and upregulates Bax expression， but may not always be necessary for apoptosis.

应用类型

Western blotting （0.5-4 ug/ml）， immunoprecipitation （4-8 ug/ml）， Immunohistochemistry （4-8 ug/ml）， immunocytochemistry （4-8 ug/ml）. However， the optimal conditions should be determined individually.

免疫原

Synthetic peptide corresponding to residue surrounding Ser20 of human p53

来源宿主

小鼠

反应性

经检测验证，可与人、小鼠、大鼠种属来源的p53蛋白发生免疫反应.

保存建议

提供的p53抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有1 mg/ml BSA和1.5 mM 叠氮钠和50%甘油.的PBS缓冲液中.请将该p53抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.