线粒体呼吸链复合体Ⅰ活性检测试剂盒(微量法)-CheKine^TM

亚科因生物研发的CheKine^TM 线粒体呼吸链复合体Ⅰ活性检测试剂盒（微量法）是一款基于比色法的微量检测工具，可快速、准确地测定植物组织、动物组织及培养细胞等样本中线粒体呼吸链复合体Ⅰ（NADH-CoQ 还原酶）的酶活性，为细胞代谢与氧化磷酸化研究提供可靠数据。

线粒体呼吸链复合体Ⅰ（EC 1.6.5.3），又称 NADH-CoQ 还原酶或 NADH 脱氢酶，是线粒体内膜上最大的蛋白复合物，广泛存在于动物、植物、微生物及培养细胞中。该酶催化 NADH 将一对电子传递给辅酶 Q（CoQ），同时使 O₂ 还原生成超氧阴离子（O₂·⁻），成为呼吸电子传递链（ETC）中活性氧（ROS）生成的主要位点。通过测定复合体Ⅰ活性，可同时评估 ETC 功能状态与 ROS 生成水平，为研究细胞能量代谢、氧化应激及相关疾病机制提供关键指标。

应用领域

适用于细胞代谢、氧化磷酸化、线粒体功能评估、活性氧（ROS）生成机制研究，以及药物筛选、疾病模型构建等细胞分析场景。

用户使用线粒体呼吸链复合体Ⅰ活性检测试剂盒(微量法)-CheKine^TM已发表的部分文献

Aberrant activated Notch1 promotes prostate enlargement driven by androgen signaling via disrupting mitochondrial function in mouse.
Journal：Cellular and Molecular Life Sciences
IF：6

Cardiomyocyte-derived USP13 protects hearts from hypertrophy via deubiquitinating and stabilizing STAT1 in male mice.
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IF：15.700

Chemical activation of mitochondrial ClpP to modulate energy metabolism of CD4+ T cell for inflammatory bowel diseases treatment.
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IF：12

CircSpna2 attenuates cuproptosis by mediating ubiquitin ligase Keap1 to regulate the Nrf2‐Atp7b signalling axis in depression after traumatic brain injury in a mouse model.
Journal：Clinical and Translational Medicine
IF：8

Divalent metal ions enhance bone regeneration through modulation of nervous systems and metabolic pathways.
Journal：Bioactive Materials
IF：20.300

ECHS1-NOX4 interaction suppresses rotenone-induced dopaminergic neurotoxicity through inhibition of mitochondrial ROS production.
Journal：Free Radical Biology and Medicine
IF：8.200

Fut2 deficiency promotes intestinal stem cell aging by damaging mitochondrial functions via down-regulating α1, 2-fucosylation of Asah2 and Npc1.
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IF：8

H2O2 negatively regulates aluminum resistance via oxidation and degradation of the transcription factor STOP1.
Journal：The Plant Cell
IF：10

Infection and chronic disease activate a systemic brain-muscle signaling axis.
Journal：Science immunology
IF：18

Interleukin-10: a novel metabolic inducer of macrophage differentiation and subsequently contributing to improved pregnancy outcomes of mice by orchestrating oxidative phosphorylation metabolism.
Journal：Biology of Reproduction
IF：3