超氧化物歧化酶(SOD)活性检测试剂盒(微量法)-CheKine^TM

CheKine^TM 超氧化物歧化酶（SOD）活性检测试剂盒（微量法）是一款基于WST-8显色反应的微量比色试剂盒，可在96孔板中快速、定量地测定细胞、组织及多种生物体液中的SOD酶活性，为细胞代谢与氧化应激研究提供可靠工具。

超氧化物歧化酶（SOD，EC 1.15.1.1）是清除超氧阴离子自由基（O₂⁻）的关键抗氧化酶，催化其歧化为O₂与H₂O₂。SOD活性异常与肌萎缩侧索硬化、神经退行性疾病、癌症及围产期致死等病理过程密切相关。人体存在三种亚型：胞质Cu/Zn-SOD（SOD1）、线粒体Mn-SOD（SOD2）和胞外Cu/Zn-SOD（SOD3）。

本试剂盒采用改良WST-8法：黄嘌呤/黄嘌呤氧化酶体系产生O₂⁻，WST-8被O₂⁻还原生成水溶性甲臜（橙色，450 nm）。SOD通过竞争性抑制WST-8还原，其活性与450 nm处吸光度下降呈线性关系，最大抑制率接近100%，且对常见干扰物（如酚红、血清蛋白）耐受性良好。

应用领域

• 细胞代谢研究：监测氧化应激模型（如H₂O₂、药物处理）中SOD活性变化。
• 疾病机制探索：评估神经退行性疾病、肿瘤或衰老模型中的抗氧化防御状态。
• 药物筛选：高通量评价抗氧化剂或候选药物对SOD活性的调节作用。
• 植物抗逆研究：分析干旱、盐胁迫等条件下植物组织的抗氧化响应。

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