线粒体膜电位分析试剂盒(JC-1法)

亚科因生物推出的线粒体膜电位分析试剂盒（JC-1法）（Mitochondrial Membrane Potential Assay Kit, JC-1）是一款专为细胞状态评估设计的核心工具，利用JC-1阳离子荧光染料快速、直观地监测线粒体膜电位变化，从而判断细胞健康或早期凋亡状态。

线粒体膜电位（ΔΨm）的下降是细胞凋亡或周期紊乱最早发生的分子事件之一。JC-1是一种亲脂性阳离子染料，在正常线粒体中因膜电位高而聚集成红色荧光聚合物；当膜电位降低时，JC-1以单体形式存在，发出绿色荧光。通过红/绿荧光强度比值即可定量评估线粒体膜电位变化，适用于流式细胞仪或荧光显微镜平台。试剂盒附带CCCP（羰基氰酸间氯苯腙）作为阳性对照，可快速诱导线粒体膜电位耗散，验证体系有效性。

应用领域

适用于细胞凋亡/周期研究、药物毒性筛选、线粒体功能评估等细胞生物学实验，尤其适合对细胞健康状态进行快速、高通量分析。

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