正常山羊血清

注意：此产品规格为10ml×10，非单一100ml。

正常山羊血清 (Normal Goat Serum) 是一种从未经免疫的健康成年山羊体内采集血液、经脂质萃取与透析精制而成的封闭试剂，专为免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等蛋白检测实验设计，用于有效降低背景信号、提高靶标抗体特异性。

在免疫荧光、免疫组化或Western blot等实验中，组织切片或细胞表面存在大量可与抗体非特异性结合的位点，导致高背景染色。正常山羊血清通过提供大量非免疫来源的山羊蛋白，可抢先占据这些非特异结合位点，从而显著降低后续一抗、二抗的非特异吸附。本产品采自非免疫山羊，经脂质萃取去除脂类干扰，再以含0.05 %叠氮化钠的PBS透析，确保澄清透明；使用时只需用PBS按1:5–1:20稀释成5 %–20 %工作液，37 °C孵育10–30 min即可完成封闭步骤，随后无需冲洗即可直接加入一抗，操作简洁。

应用领域

适用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)、酶联免疫吸附(ELISA)及封闭印迹(BL)等实验中组织切片、细胞爬片、膜转印样本的非特异性位点封闭，特别适合与山羊来源二抗配套使用，以提升信噪比与实验重现性。

用户使用正常山羊血清已发表的部分文献

Deubiquitinase Ubiquitin‐Specific Protease 29 Ameliorates Pathological Cardiac Hypertrophy through Inhibiting Transforming Growth Factor β‐Activated Kinase 1.
Journal：Journal of the American Heart Association
IF：5.300

Enhanced CXCL10 expression in mast cells for cutaneous neurofibroma presenting with pain and itch.
Journal：British Journal of Cancer
IF：6.800

Genetic deletion of zinc transporter ZnT3 induces progressive cognitive deficits in mice by impairing dendritic spine plasticity and glucose metabolism.
Journal：Frontiers in Molecular Neuroscience
IF：4

Inflammation-driven biomimetic nano-polyphenol drug delivery system alleviates severe acute pancreatitis by inhibiting macrophage PANoptosis and pancreatic enzymes oversecretion.
Journal：Journal of Advanced Research
IF：13.000

Nocardia cyriacigeorgica Elicits Gut Disturbances in a Leaky Gut Model of Colitis, but Not the Harmful Cascade Leading to Gut-First Parkinson’s Disease.
Journal：International Journal of Molecular Sciences
IF：5

Sertraline alleviates chronic prostatitis by regulating the TRPV1 channel.
Journal：Journal of Inflammation Research
IF：4

TMEM100 acts as a TAK1 receptor that prevents pathological cardiac hypertrophy progression.
Journal：Cell Communication and Signaling
IF：8