甲基赖氨酸抗体，琼脂糖

详细介绍

Methyl Lysine Antibody, Agarose

甲基赖氨酸抗体，琼脂糖

Catalog#目录号:ICP0588

Product Description产品描述:

The methylated lysine antibodies are developed with methylated KLH and affinity purified with methylated lysine on agarose. The purified antibodies are covalently immobilized to beaded agarose. The product could be utilized as an affinity matrix for rapid isolation, purification, and enrichment of the proteins or peptide with methylted lysine residues, particularly trimethyl- and/or dimethyl-lysine residues.

甲基化赖氨酸抗体采用甲基化KLH开发，并通过琼脂糖上的甲基化赖氨酸进行亲和纯化。纯化后的抗体共价固定在琼脂糖珠上。该产品可用作亲和基质，用于快速分离、纯化和富集含有甲基化赖氨酸残基（特别是三甲基和/或二甲基赖氨酸残基）的蛋白质或多肽。

Formulation配方:

0.5 ml beaded agarose suspended in 1 ml slurry with glycerol

0.5 ml 琼脂糖珠悬浮在 1 ml 含甘油的浆液中

Antibody Immobilized抗体固定

10 mg/ml of the methylated lysine antibodies are covalently linked to the agarose beads

10 mg/ml 的甲基化赖氨酸抗体共价结合到琼脂糖珠上

Specificity特异性:

This antibody matrix selectively captures the peptides and proteins with methylated lysine residues (N-epsilon).

该抗体基质可选择性捕获带有甲基化赖氨酸残基（N-epsilon）的肽和蛋白质。

Binding Capacity结合容量:

Approximately 0.5 mg of methylated histone/ml

约0.5 mg甲基化组蛋白/ml

Applications应用:

Rapid isolation, purification, and affinity enrichment of peptides or proteins with the methylated lysine residues from mixtures of cell lysates or protease-digested samples.

从细胞裂解物混合物或蛋白酶消化样本中快速分离、纯化并亲和富集含有甲基化赖氨酸残基的肽或蛋白质。

Scientific Description科学描述:

Protein methylation is a form of post-translational modification known to regulate many diverse biological processes. Detection, isolation and identification of the methylated protein/peptide is essential in proteomic studies. Affinity chromatography is one of the most efficient and rapid methods to enrich and purify the methylated species for further MS/MS identification.

蛋白质甲基化是一种已知的调节多种生物过程的翻译后修饰形式。在蛋白质组学研究中，检测、分离和鉴定甲基化蛋白质/多肽至关重要。亲和层析是富集和纯化甲基化物种以进行进一步质谱/质谱鉴定最有效且快速的方法之一。

Storage & Stability储存与稳定性:

Product is stable for 30 days at room temperature. For extended storage, store product at -20°C. Do not aliquot and shake thoroughly before use. Expiration date is one year from date of shipping of properly stored.

产品在室温下可稳定保存30天。如需长期储存，请将产品存放在-20°C。请勿分装，使用前需充分摇匀。在妥善储存的情况下，有效期为发货之日起一年。