一、产品概述
Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody 是来自CST品牌的高品质产品，适用于科研实验领域。
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二、产品信息
| 货号 | 2601 |
| SKU货号 | 2601S |
| 品牌 | CST |
| 中文名称 | Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody |
| 英文名称 | Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody |
| 分子量 | 61 to 67 (PAK2), 68 to 74 (PAK1/3) |
| 抗原名称 | PAK1PAK2 |
| 来源纯化 | Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr423 of human PAK1. Antibodies are purified by protein A and peptide affinity chromatography. |
商品描述
Product Usage Information
| Application | Dilution |
|---|
| Western Blotting | 1:1000 |
三、背景
背景
Background
The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, control of phagocyte NADPH oxidase, and growth factor-induced neurite outgrowth (1,2). Several mechanisms that induce PAK activity have been reported. Binding of Rac/Cdc42 to the CRIB (or PBD) domain near the amino terminus of PAK causes autophosphorylation and conformational changes in PAK (1). Phosphorylation of PAK1 at Thr423 by PDK induces activation of PAK1 (3). Several autophosphorylation sites have been identified, including Ser199 and Ser204 of PAK1, and Ser192 and Ser197 of PAK2 (4,5). Because the autophosphorylation sites are located in the amino-terminal inhibitory domain, it has been hypothesized that modification in this region prevents the kinase from reverting to an inactive conformation (6). Research indicates that phosphorylation at Ser144 of PAK1 or Ser139 of PAK3 (located in the kinase inhibitory domain) affects kinase activity (7). Phosphorylation at Ser21 of PAK1 or Ser20 of PAK2 regulates binding with the adaptor protein Nck (8). PAK4, PAK5/7, and PAK6 have lower sequence similarity with PAK1-3 in the amino-terminal regulatory region (9). Phosphorylation at Ser474 of PAK4, a site analogous to Thr423 of PAK1, may play a pivotal role in regulating the activity and function of PAK4 (10). PAK family members are widely expressed, and often overexpressed in human cancer (11,12).
- Knaus, U.G. and Bokoch, G.M. (1998) Int. J. Biochem. Cell Biol. 30, 857-62.
- Daniels, R.H. et al. (1998) EMBO J. 17, 754-64.
- King, C.C. et al. (2000) J. Biol. Chem. 275, 41201-9.
- Manser, E. et al. (1997) Mol. Cell. Biol. 17, 1129-43.
- Gatti, A. et al. (1999) J. Biol. Chem. 274, 8022-8.
- Lei, M. et al. (2000) Cell 102, 387-97.
- Chong, C. et al. (2001) J. Biol. Chem. 276, 17347-53.
- Zhao, Z. et al. (2000) Mol. Cell. Biol. 20, 3906-17.
- Abo, A. et al. (1998) EMBO J. 17, 6527-40.
- Qu, J. et al. (2001) Mol. Cell. Biol. 21, 3523-33.
- Wen, Y.Y. et al. (2014) Expert Opin Ther Targets 18, 807-15.
- Molli, P.R. et al. (2009) Oncogene 28, 2545-55.
Alternate Names
adriamycin resistance-associated; Alpha-PAK; ARA; Beta-PAK; bPAK; C-t-PAK2; CDKN1A; Gamma-PAK; hPAK3; IDDMSSD; MGC130000; MGC130001; MRX30; MRX47; Oligophrenin-3; OPHN3; p21 (CDKN1A)-activated kinase 2; p21 (CDKN1A)-activated kinase 3; p21 (RAC1) activated kinase 1; p21 (RAC1) activated kinase 2; p21 (RAC1) activated kinase 3; p21 protein (Cdc42/Rac)-activated kinase 1; p21 protein (Cdc42/Rac)-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 3; p21-activated kinase 1; p21-activated kinase 2; p21-activated kinase 3; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p27; p34; p58; p65-PAK; PAK-1; PAK-2; PAK-2p27; PAK-2p34; PAK-3; PAK1; PAK2; PAK3; PAK3beta; PAK65; PAKalpha; PAKgamma; S6/H4 kinase; Serine/threonine-protein kinase PAK 1; Serine/threonine-protein kinase PAK 2; Serine/threonine-protein kinase PAK 3; STE20 homolog, yeast
四、制备和贮存
| 保存方式 |
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
|
五、应用
| 反应种属 | Human,Mouse,Pig |
| 敏感性 | Endogenous |
| 目标/特异性 | Specificity / Sensitivity Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody detects endogenous PAK1, PAK2 and PAK3 only when phosphorylated at Thr423, Thr402 and Thr421, respectively. This antibody does not cross-react with phosphorylated PAK4, PAK5 or PAK6. The antibody does cross-react with phospho-Mst1 (Thr183) or phospho-Mst2 (Thr180). Species Reactivity: Human, Mouse, Guinea Pig
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here,
but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not
covered under our
Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Rat |
六、产品特点
- 品质保证：经过严格的质量控制，确保产品的高品质和稳定性。
七、注意事项
- 本产品仅供科学研究使用，不可用于临床诊断或治疗。
- 请在通风良好的环境下操作，佩戴适当的个人防护装备。
- 如不慎接触皮肤或眼睛，请立即用大量清水冲洗并就医。
