可固定活性染色试剂575V-565694

一、产品概述

二、产品信息

BD Horizon Fixable Viability Stain 575V (FVS575V) is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. This dye reacts with and covalently binds to cell-surface and intracellular amines. Permeable plasma cell membranes, such as those present in necrotic cells, allow for the intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines than in nonpermeable live cells. Therefore, necrotic cells present in a typical
in vitro
assay label with higher levels of dye increasing their fluorescence intensity 10-20 fold over that of viable cells. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.
BD Horizon Fixable Viability Stain 575V is excited by the Violet laser (with an excitation maximum of 396 nm), and has a fluorescence emission maximum of 572 nm.
Danger:
Hazard statement
:
Causes serious eye damage.
Precautionary statements
:
Wear eye protection / face protection. Wash thoroughly after handling.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.  Immediately call a POISON CENTER/doctor.
Disposal:
Dispose of contents/container to an appropriate treatment and disposal facility in accordance with applicable laws and regulations, and product characteristics at time of disposal.

BD Horizon Fixable Viability Stain 575V (FVS575V) is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. This dye reacts with and covalently binds to cell-surface and intracellular amines. Permeable plasma cell membranes, such as those present in necrotic cells, allow for the intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines than in nonpermeable live cells. Therefore, necrotic cells present in a typical
in vitro
assay label with higher levels of dye increasing their fluorescence intensity 10-20 fold over that of viable cells. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.
BD Horizon Fixable Viability Stain 575V is excited by the Violet laser (with an excitation maximum of 396 nm), and has a fluorescence emission maximum of 572 nm.
Danger:
Hazard statement
:
Causes serious eye damage.
Precautionary statements
:
Wear eye protection / face protection. Wash thoroughly after handling.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.  Immediately call a POISON CENTER/doctor.
Disposal:
Dispose of contents/container to an appropriate treatment and disposal facility in accordance with applicable laws and regulations, and product characteristics at time of disposal.

三、应用

四、产品特点

五、注意事项