人 Granzyme B 一步法 ELISA 试剂盒-S0C3041

Granzyme B (GrB) is a serine protease pivotal in cytotoxic immune responses, mediating apoptosis and immune surveillance. Structurally, GrB is synthesized as a 32 kDa inactive pro-enzyme (zymogen) and activated through proteolytic cleavage. Its active form features a conserved catalytic triad (His57, Asp102, Ser195) essential for enzymatic activity. Post-translational modifications, including N-linked glycosylation, enhance its stability and regulate intracellular trafficking. GrB functions by binding to mannose-6-phosphate receptors (MPRs) for endosomal uptake into target cells, where it synergizes with perforin to induce cell death.

Advantages of this OneStep ELISA Kit Design:

Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time.

High Efficiency: Achieves results within 60 minutes, ideal for high-throughput studies or urgent diagnostics.

Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation.

Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design.

This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable Granzyme B quantification in time-sensitive or resource-limited settings.