Thalidomide-O-PEG4-NHS ester

Thalidomide-O-PEG4-NHS ester is a PEG-based E3 ligase ligand-linker conjugate designed for use in the synthesis of PROTACs and other targeted protein degradation molecules. The compound contains a thalidomide-derived Cereblon (CRBN) ligand connected to a flexible PEG4 linker and a terminal N-hydroxysuccinimide (NHS) ester. The NHS ester provides a reactive functional group for coupling with primary amines, enabling the efficient incorporation of the CRBN-recruiting moiety into heterobifunctional PROTAC architectures.

The chemical name of Thalidomide-O-PEG4-NHS ester is 2,5-dioxopyrrolidin-1-yl 1-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)-3,6,9,12-tetraoxapentadecan-15-oate. It has a CAS No. of 2411681-88-2, molecular formula C₂₈H₃₃N₃O₁₃, and molecular weight 619.57 g/mol.

Thalidomide-O-PEG4-NHS ester is useful as a building block in medicinal chemistry, chemical biology, and targeted protein degradation research. Its PEG4 spacer can provide flexibility and appropriate distance between the CRBN ligand and the protein-targeting ligand, while the NHS ester facilitates amide-bond formation with amine-containing compounds. This makes the compound suitable for developing CRBN-recruiting PROTACs and related molecular degraders. For research use only.