Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Shipping with blue ice/Shipping at ambient temperature.
产品类别：
抑制剂
货号：
T001|T200009
Product Introduction
Bioactivity
名称
Pregnant mare serum gonadotropin
描述
Pregnant mare serum gonadotropin (PMSG) is a glycoprotein gonadotropin derived from the serum of pregnant mares. It exhibits FSH/LH-like activity and promotes follicular development and ovulation in animals. Its effects are primarily mediated through the regulation of endocrine signaling along the hypothalamic–pituitary–gonadal axis, as well as by promoting the proliferation of ovarian granulosa cells and the secretion of estrogen, thereby influencing the progression of the reproductive cycle. In livestock production, PMSG is commonly used to induce estrus and improve reproductive efficiency; it is also widely used in research on reproductive endocrinology and the mechanisms regulating the estrous cycle.
体内活性
方法： 23日龄雌性Holtzman大鼠于30日龄上午10:00皮下注射4–64 IU Pregnant mare serum gonadotropin (PMSG)，在注射后不同时间点（8、24、30、48、54、72、95 h及35–39日龄）处死，称量卵巢和子宫湿重，采集血液，用RIA检测血清类固醇和促性腺激素水平，并测定下丘脑和垂体前叶胞质雌二醇受体含量。
结果： PMSG（4–64 IU；皮下注射；单次给药）可诱导未成熟雌性Holtzman大鼠完全排卵，触发血清类固醇、促性腺激素及下丘脑-垂体胞浆雌二醇受体的周期性变化，模拟成年大鼠的动情周期，并可引起卵巢和子宫重量呈剂量依赖性增加（8 IU为最小有效剂量）[1]。
方法： 给雌性山羊肌肉注射1200 IU PMSG（单次给药），分别在给药后不同时间点采集血液样本，使用大鼠Leydig细胞生物测定法检测血浆中促黄体生成素 (LH) 的生物活性。
结果： PMSG（1200 IU；肌肉注射；单次给药）给药后4-8小时内，血浆中具有生物活性的LH水平升高至接近LH峰浓度（>5 ng/mL），并持续升高超过72小时；而同期放射免疫法测得的免疫反应性LH始终低于1.5 ng/mL，表明PMSG分子本身具有内在的LH生物活性，可直接诱导排卵[2]。
存储条件
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Shipping with blue ice/Shipping at ambient temperature.
