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Mouse Anti DDK/Flag mAb Description

The DYKDDDDK(Flag)-tagged expression system is widely used in the production of recombinant protein. This is a high-affinity monoclonal antibody that can be used for detection of DYKDDDDK(Flag)-tagged proteins.

Host Species

Mouse

Immunogen

KLH coupled synthetic peptide DYKDDDDK.

Ig Subclass

IgG2b, k

Purification

Protein A affinity chromatograph.

Specificity

recognizes C-terminal, N-terminal, and internal DYKDDDDK(Flag) tagged fusion proteins.

Applications

ELISA: 1:5000 WB: 1:1000 IP: 1:500 IF: 1:500 FACS: 1:500

Other applications: user-optimized.

Concentration

0.5 mg/ml with stabilizer(1%BSA,5%Sucrose,etc.) pH 7.4, containing 0.02% thimerosal.

Storage

stored for up to six months at 2-8°C or for up to two years at -20°C or below.

Avoid repeated freeze and thaw cycles.