抗体 Anti-BRD2 Antibody (3E992)|TMAB-03213|TargetMol

中文名称：

抗体 Anti-BRD2 Monoclonal Antibody (3E992)

英文名称：

Anti-BRD2 Monoclonal Antibody (3E992)

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature -20°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Recombinant Monoclonal Antibody

种属：

Human

应用：

WB,IHC-P,ICC/IF,FCM

反应性：

Human

宿主：

Rabbit

偶联物：

Unconjugated

靶点：

BRD2

克隆性：

Monoclonal

免疫原：

Synthetic peptide of human BRD2

亚型：

IgG

验证方法：

1. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BRD2  Monoclonal Antibody, Unconjugated (TMAB-03213) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 2. 25 ug total protein per lane of various lysates (see on figure) probed with BRD2 monoclonal antibody, unconjugated (TMAB-03213) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 3. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-BRD2 antibody (TMAB-03213, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed. 4. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (BRD2) monoclonal Antibody, unconjugated (TMAB-03213) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

UniProt ID：

P25440

克隆号：

3E992

浓度：

1mg/ml

基因名称：

BRD2

货号：

T014|TMAB-03213