血小板源性生长因子BB抗体|TMAB-01353|TargetMol

中文名称：

抗体 Anti-PDGFB Polyclonal Antibody 2

英文名称：

Anti-PDGFB Polyclonal Antibody 2

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

WB,IHC-P,ICC/IF,ELISA

反应性：

Human,Mouse,Rat

宿主：

Rabbit

偶联物：

Unconjugated

靶点：

PDGFB

克隆性：

Polyclonal

免疫原：

The antiserum was produced against synthesized peptide derived from human PDGFB.

亚型：

IgG

验证方法：

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PDGFBB) Polyclonal Antibody, Unconjugated (TMAB-01353) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-PDGF-BB Polyclonal Antibody, Unconjugated (TMAB-01353) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-PDGF-BB Polyclonal Antibody, Unconjugated (TMAB-01353) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 4. Sample: Lane 1: Huvec (Human) Cell Lysate at 30 μg Lane 2: A431 (Human) Cell Lysate at 30 μg Lane 3: MCF-7 (Human) Cell Lysate at 30 μg Primary: Anti-PDGFBB (TMAB-01353) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27/22/14 kDa Observed band size: 30 kDa

UniProt ID：

P01127

浓度：

1 mg/mL

基因名称：

PDGFB

货号：

T014|TMAB-01353