抗体 Anti-eIF4A3 Antibody (1Y205)|TMAB-05515|TargetMol

中文名称：

抗体 Anti-eIF4A3 Monoclonal Antibody (1Y205)

英文名称：

Anti-eIF4A3 Monoclonal Antibody (1Y205)

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature -20°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Recombinant Monoclonal Antibody

种属：

Human

应用：

WB,IHC-F,IHC-P,ICC/IF,FCM,IP

反应性：

Human,Mouse,Rat

宿主：

Rabbit

偶联物：

Unconjugated

靶点：

EIF4A3

克隆性：

Monoclonal

免疫原：

Synthetic peptide of human Eif4a3

亚型：

IgG

验证方法：

1. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (eIF4A3) monoclonal Antibody, unconjugated (TMAB-05515) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control. 2. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF4A3 Monoclonal Antibody, Unconjugated (TMAB-05515) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF4A3 Monoclonal Antibody, Unconjugated (TMAB-05515) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF4A3 Monoclonal Antibody, Unconjugated (TMAB-05515) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF4A3 Monoclonal Antibody, Unconjugated (TMAB-05515) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF4A3 Monoclonal Antibody, Unconjugated (TMAB-05515) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF4A3 Monoclonal Antibody, Unconjugated (TMAB-05515) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF4A3 Monoclonal Antibody, Unconjugated (TMAB-05515) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF4A3 Monoclonal Antibody, Unconjugated (TMAB-05515) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF4A3 Monoclonal Antibody, Unconjugated (TMAB-05515) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 11. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF4A3 Monoclonal Antibody, Unconjugated (TMAB-05515) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 12. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF4A3 Monoclonal Antibody, Unconjugated (TMAB-05515) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 13. Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF4A3 Monoclonal Antibody, Unconjugated (TMAB-05515) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.

UniProt ID：

P38919

克隆号：

1Y205

基因名称：

EIF4A3

货号：

T014|TMAB-05515