抗体 Anti-EGR2 Polyclonal Antibody|TMAB-05475|TargetMol

中文名称：

抗体 Anti-EGR2 Polyclonal Antibody

英文名称：

Anti-EGR2 Polyclonal Antibody

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature -20°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

WB,IHC-P,IHC-Fr,IF

反应性：

Human,Mouse (predicted:Rat)

宿主：

Rabbit

靶点：

EGR2

克隆性：

Polyclonal

免疫原：

KLH conjugated synthetic peptide: human EGR2

亚型：

IgG

验证方法：

1. Sample: U87mg (Mouse) Lysate at 40 μg Primary: Anti-EGR2 (TMAB-05475) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD 2. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (EGR2) Polyclonal Antibody, Unconjugated (TMAB-05475) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Sample: HepG2 (Human) Cell Lysate at 30 μg MCF-7 (Human) Cell Lysate at 30 μg Primary: Anti-EGR2 (TMAB-05475) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 52 kD

UniProt ID：

P11161

浓度：

1mg/ml

基因名称：

EGR2

货号：

T014|TMAB-05475