磷酸化信号转导和转录激活因子3抗体|TMAB-01510|TargetMol

中文名称：

磷酸化信号转导和转录激活因子3抗体

英文名称：

Anti-Phospho-STAT3 (Tyr705) Polyclonal Antibody 2

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

IF,IHC-Fr,IHC-P

反应性：

Human,Mouse,Rat

宿主：

Rabbit

靶点：

STAT3

克隆性：

Polyclonal

免疫原：

KLH conjugated Synthesised phosphopeptide: human STAT3 around the phosphorylation site of Tyr705

亚型：

IgG

验证方法：

1. Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-STAT3 (Tyr705)) Polyclonal Antibody, Unconjugated (TMAB-01510) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT3 (Tyr705) alpha Polyclonal Antibody, Unconjugated (TMAB-01510) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT3 (Tyr705) alpha Polyclonal Antibody, Unconjugated (TMAB-01510) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT3 (Tyr705) alpha Polyclonal Antibody, Unconjugated (TMAB-01510) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT3 (Tyr705) alpha Polyclonal Antibody, Unconjugated (TMAB-01510) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT3 (Tyr705) alpha Polyclonal Antibody, Unconjugated (TMAB-01510) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

UniProt ID：

P40763

浓度：

1 mg/mL

基因名称：

STAT3

货号：

T014|TMAB-01510