抗体 Anti-BCL11A Antibody (7U151)|TMAB-03048|TargetMol

中文名称：

抗体 Anti-BCL11A Monoclonal Antibody (7U151)

英文名称：

Anti-BCL11A Monoclonal Antibody (7U151)

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature -20°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Recombinant Monoclonal Antibody

种属：

Human

应用：

WB,IHC-P,ICC/IF,FCM,IP

反应性：

Human,Mouse,Rat

宿主：

Rabbit

偶联物：

Unconjugated

靶点：

BCL11A

克隆性：

Monoclonal

免疫原：

Recombinant protein of human Ctip1

亚型：

IgG

验证方法：

1. 25 μg total protein per lane of various lysates (see on figure) probed with BCL11A monoclonal antibody, unconjugated (TMAB-03048) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. The Jurkat (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-BCL11A antibody (TMAB-03048, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed. 3. 4% Paraformaldehyde-fixed Jurkat (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (BCL11A) monoclonal Antibody, unconjugated (TMAB-03048) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

UniProt ID：

Q9H165

克隆号：

7U151

基因名称：

BCL11A

货号：

T014|TMAB-03048