磷酸化核因子2相关因子2(Ser40)重组兔单抗|TMAB-01463|TargetMol

中文名称：

磷酸化核因子2相关因子2(Ser40)重组兔单抗

英文名称：

Anti-Phospho-NFE2L2 (Ser40) Antibody (8Z908)

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Recombinant Antibody

种属：

Human

应用：

FCM,ICC/IF,IF,IHC-Fr,IHC-P,WB

反应性：

Human

宿主：

Rabbit

靶点：

NFE2L2

克隆性：

Monoclonal

免疫原：

A synthesized peptide: human Nrf2 around the phosphorylation site of S40

亚型：

IgG

验证方法：

1. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (phospho-Nrf2 (Ser40)) monoclonal Antibody, Unconjugated (TMAB-01463) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 2. Blank control: Hela. Primary Antibody (green line): Rabbit Anti-phospho-Nrf2 (Ser40) antibody (TMAB-01463) Dilution: 1 μg/Test; Secondary Antibody: Goat anti-rabbit IgG-BF488 Dilution: 0.5 μg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 3. 25 μg total protein per Lane of various lysates probed with phospho-Nrf2 (Ser40) monoclonal antibody, unconjugated (TMAB-01463) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 4. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-Nrf2 (Ser40) Monoclonal Antibody, Unconjugated (TMAB-01463) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-Nrf2 (Ser40) Monoclonal Antibody, Unconjugated (TMAB-01463) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-Nrf2 (Ser40) Monoclonal Antibody, Unconjugated (TMAB-01463) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Skin Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-Nrf2 (Ser40) Monoclonal Antibody, Unconjugated (TMAB-01463) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-Nrf2 (Ser40) Monoclonal Antibody, Unconjugated (TMAB-01463) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

UniProt ID：

Q16236

克隆号：

8Z908

浓度：

1 mg/mL

基因名称：

NFE2L2

货号：

T014|TMAB-01463