磷酸化组蛋白H2AX重组兔单抗|TMAB-01419|TargetMol

中文名称：

磷酸化组蛋白H2AX重组兔单抗

英文名称：

Anti-Phospho-H2AFX (Ser139) Antibody (2T270)

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Recombinant Antibody

种属：

Human

应用：

WB,IHC-P,IHC-Fr,IF

反应性：

Human,Mouse,Rat

宿主：

Rabbit

靶点：

H2AX

克隆性：

Monoclonal

免疫原：

A synthesized peptide: human Histone H2A.X around the phosphorylation site of S139

亚型：

IgG

验证方法：

1. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (TMAB-01419) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (TMAB-01419) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (TMAB-01419) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (TMAB-01419) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (TMAB-01419) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Rat Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (TMAB-01419) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (TMAB-01419) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (TMAB-01419) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (TMAB-01419) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (TMAB-01419) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 11. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (TMAB-01419) at 1:800 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 12. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (TMAB-01419) at 1:800 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 13. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (TMAB-01419) at 1:800 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 14. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-H2AX (Ser139) Monoclonal Antibody, Unconjugated (TMAB-01419) at 1:800 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 15. Jurkat (H) cells were treated with UV for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-H2AX (Ser139) monoclonal antibody, unconjugated (TMAB-01419) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

UniProt ID：

P16104

克隆号：

2T270

浓度：

1 mg/mL

基因名称：

H2AX

货号：

T014|TMAB-01419