磷酸化内质网核信号转导蛋白a1抗体|TMAB-01433|TargetMol

中文名称：

磷酸化内质网核信号转导蛋白a1抗体

英文名称：

Anti-Phospho-IRE1 (Ser726) Polyclonal Antibody

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

FCM,IF,IHC-Fr,IHC-P

反应性：

Human,Rat (predicted:Mouse,Dog,Pig,Cow,Horse,Rabbit)

宿主：

Rabbit

靶点：

ERN1

克隆性：

Polyclonal

免疫原：

KLH conjugated synthesised phosphopeptide derived from human IRE1a around the phosphorylation site of Ser726

亚型：

IgG

验证方法：

1. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IRE1a (Ser 726)) Polyclonal Antibody, Unconjugated (TMAB-01433) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Blank control: Raji. Primary Antibody (green line): Rabbit Anti-phospho-IRE1a (Ser 726) antibody (TMAB-01433) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

UniProt ID：

O75460

浓度：

1 mg/mL

基因名称：

ERN1

货号：

T014|TMAB-01433