磷酸化钙/钙调素依赖蛋白激酶2α抗体|TMAB-01398|TargetMol

中文名称：

磷酸化钙/钙调素依赖蛋白激酶2α抗体

英文名称：

Anti-Phospho-CaMK2 alpha (Tyr230) Polyclonal Antibody

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

FCM,IF,IHC-Fr,IHC-P,WB

反应性：

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)

宿主：

Rabbit

靶点：

CAMK2A

克隆性：

Polyclonal

免疫原：

KLH conjugated Synthesised phosphopeptide derived from human CaMK2 alpha around the phosphorylation site of Tyr231

亚型：

IgG

验证方法：

1. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p-CaMK2 alpha (Tyr230)) Polyclonal Antibody, Unconjugated (TMAB-01398) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p-CaMK2 alpha (Tyr230)) Polyclonal Antibody, Unconjugated (TMAB-01398) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p-CaMK2 alpha (Tyr230)) Polyclonal Antibody, Unconjugated (TMAB-01398) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Sample: Cerebrum (Rat) cell Lysate at 40 μg Primary: Anti-phospho-CaMK2 alpha (Tyr230) (TMAB-01398) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa 5. Blank control: K562. Primary Antibody (green line): Rabbit Anti-phospho-CaMK2 alpha (Tyr230) antibody (TMAB-01398) Dilution: 2 μg/Test; Secondary Antibody: Goat anti-rabbit IgG-FITC Dilution: 0.5 μg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 6. Paraformaldehyde-fixed, paraffin embedded (Mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p-CaMK2 alpha) Polyclonal Antibody, Unconjugated (TMAB-01398) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 7. Sample: Lane 1: Mouse Muscle tissue lysates Lane 2: Mouse Heart tissue lysates Primary: Anti-phospho-CaMK2 alpha (Tyr230) (TMAB-01398) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 50 kDa

UniProt ID：

Q9UQM7

浓度：

1 mg/mL

基因名称：

CAMK2A

货号：

T014|TMAB-01398