磷酸化蛋白激酶AKT3抗体|TMAB-01384|TargetMol

中文名称：

磷酸化蛋白激酶AKT3抗体

英文名称：

Anti-Phospho-AKT3 (Ser472) Polyclonal Antibody

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

IF,IHC-Fr,IHC-P,WB

反应性：

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Rabbit,Sheep)

宿主：

Rabbit

靶点：

AKT3

克隆性：

Polyclonal

免疫原：

KLH conjugated Synthesised phosphopeptide: human AKT3 around the phosphorylation site of Ser472

亚型：

IgG

验证方法：

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-AKT3 (Ser472)) Polyclonal Antibody, Unconjugated (TMAB-01384) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Tissue/cell: human lung carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-phospho-AKT3 (Ser472) Polyclonal Antibody, Unconjugated (TMAB-01384) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus. 3. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-AKT3 (Ser472)) Polyclonal Antibody, Unconjugated (TMAB-01384) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Sample: Lane 1: Huvec (Human) Cell Lysate at 30 μg Lane 2: A431 (Human) Cell Lysate at 30 μg Primary: Anti-phospho-AKT3 (Ser472) (TMAB-01384) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kDa Observed band size: 58 kDa 5. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-AKT3 (Ser472)) Polyclonal Antibody, Unconjugated (TMAB-01384) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-AKT3 (Ser472)) Polyclonal Antibody, Unconjugated (TMAB-01384) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

UniProt ID：

Q9Y243

浓度：

1 mg/mL

基因名称：

AKT3

货号：

T014|TMAB-01384