抗体 Anti-Bak Antibody (3J180)|TMAB-03021|TargetMol

中文名称：

抗体 Anti-Bak Monoclonal Antibody (3J180)

英文名称：

Anti-Bak Monoclonal Antibody (3J180)

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature -20°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Recombinant Monoclonal Antibody

种属：

Human

应用：

WB,IHC-P,ICC/IF,FCM,IP

反应性：

Human

宿主：

Rabbit

偶联物：

Unconjugated

靶点：

BAK1

克隆性：

Monoclonal

免疫原：

A synthesized peptide derived from human Bak

亚型：

IgG

验证方法：

1. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bak Monoclonal Antibody, Unconjugated (TMAB-03021) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bak Monoclonal Antibody, Unconjugated (TMAB-03021) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bak Monoclonal Antibody, Unconjugated (TMAB-03021) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. 4% Paraformaldehyde-fixed THP-1 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Bak) monoclonal Antibody, unconjugated (TMAB-03021) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control. 5. The THP-1 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-Bak antibody (TMAB-03021, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed. 6. 25 ug total protein per lane of various lysates (see on figure) probed with Bak monoclonal antibody, unconjugated (TMAB-03021) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

UniProt ID：

Q16611

克隆号：

3J180

基因名称：

BAK1

货号：

T014|TMAB-03021