抗体 Anti-ADAMTS7(NT) Polyclonal Antibody|TMAB-02305|TargetMol

中文名称：

抗体 Anti-ADAMTS7(NT) Polyclonal Antibody

英文名称：

Anti-ADAMTS7(NT) Polyclonal Antibody

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature -20°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

WB,IHC-P,IHC-Fr,IF

反应性：

Mouse,Rat (predicted:Human)

宿主：

Rabbit

靶点：

ADAMTS7

克隆性：

Polyclonal

免疫原：

KLH conjugated synthetic peptide derived from human ADAMTS7

亚型：

IgG

验证方法：

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-ADAMTS7 Polyclonal Antibody, Unconjugated (TMAB-02305) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-ADAMTS7 Polyclonal Antibody, Unconjugated (TMAB-02305) 1: 200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1: 200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml, blue) was used to stain the cell nuclei 3. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-ADAMTS7 (NT) (TMAB-02305) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 158 kD Observed band size: 158 kD 4. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ADAMTS7 (NT)) Polyclonal Antibody, Unconjugated (TMAB-02305) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

UniProt ID：

Q9UKP4

浓度：

1mg/ml

基因名称：

ADAMTS7

货号：

T014|TMAB-02305