细胞毒性受体NK-p46抗体|TMAB-01241|TargetMol

中文名称：

细胞毒性受体NK-p46抗体

英文名称：

Anti-NKp46/NCR1 Polyclonal Antibody

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

FCM,IF,IHC-Fr,IHC-P,WB

反应性：

Human,Mouse

宿主：

Rabbit

靶点：

NCR1

克隆性：

Polyclonal

免疫原：

KLH conjugated synthetic peptide: human NCR1

亚型：

IgG

验证方法：

1. Paraformaldehyde-fixed, paraffin embedded (human skin cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NCR1) Polyclonal Antibody, Unconjugated (TMAB-01241) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-NCR1 antibody (TMAB-01241) Dilution: 2 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 3. Sample: A431 (Human) Cell Lysate at 30 μg Raji (Human) Cell Lysate at 30 μg Primary: Anti-NCR1 (TMAB-01241) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kDa Observed band size: 46 kDa 4. Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-NCR1 antibody (TMAB-01241) Dilution: 2 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 5. Sample: Kidney (Mouse) Lysate at 40 μg Primary: Anti-NCR1 (TMAB-01241) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kDa Observed band size: 46 kDa

UniProt ID：

O76036

浓度：

1 mg/mL

基因名称：

NCR1

货号：

T014|TMAB-01241