抗体 Anti-CNPase Antibody (5O365)|TMAB-04495|TargetMol

中文名称：

抗体 Anti-CNPase Antibody (5O365)

英文名称：

Anti-CNPase Antibody (5O365)

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature -20°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Recombinant Antibody

种属：

Human

应用：

WB,IHC-P,IHC-Fr,ICC/IF,IF

反应性：

Human,Rat

宿主：

Mouse

靶点：

CNP

克隆性：

Monoclonal

免疫原：

KLH conjugated synthetic peptide: human CNPase

亚型：

IgG1, Kappa

验证方法：

1. SHSY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (CNPase) polyclonal Antibody, Unconjugated (TMAB-04495) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. 2. Sample: Lane 1: Rat Cerebrum tissue lysates Lane 2: Rat Cerebellum tissue lysates Primary: Anti-CNPase (TMAB-04495) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 48 kDa Observed band size: 46 kDa 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CNPase Monoclonal Antibody, Unconjugated (TMAB-04495) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CNPase Monoclonal Antibody, Unconjugated (TMAB-04495) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CNPase Monoclonal Antibody, Unconjugated (TMAB-04495) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CNPase Monoclonal Antibody, Unconjugated (TMAB-04495) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining. 7. 25 μg total protein per lane of various lysates (see on figure) probed with CNPase monoclonal antibody, unconjugated (TMAB-04495) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

UniProt ID：

P09543

克隆号：

5O365

浓度：

1mg/ml

基因名称：

CNP

货号：

T014|TMAB-04495