抗体 Anti-Dnmt1 Antibody (6Q563)|TMAB-05258|TargetMol

中文名称：

抗体 Anti-Dnmt1 Monoclonal Antibody (6Q563)

英文名称：

Anti-Dnmt1 Monoclonal Antibody (6Q563)

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature -20°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Recombinant Monoclonal Antibody

种属：

Human

应用：

WB,IHC-P,ICC/IF,FCM

反应性：

Human,Mouse,Rat

宿主：

Rabbit

偶联物：

Unconjugated

靶点：

DNMT1

克隆性：

Monoclonal

免疫原：

Synthetic peptide of human Dnmt1

亚型：

IgG

验证方法：

1. Paraformaldehyde-fixed, paraffin embedded Rat testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated (TMAB-05258) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Rat placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated (TMAB-05258) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated (TMAB-05258) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human tonsils; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated (TMAB-05258) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated (TMAB-05258) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated (TMAB-05258) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated (TMAB-05258) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Mouse testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated (TMAB-05258) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated (TMAB-05258) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated (TMAB-05258) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 11. Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Dnmt1 Monoclonal Antibody, Unconjugated (TMAB-05258) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 12. 25 μg total protein per lane of various lysates (see on figure) probed with Dnmt1 monoclonal antibody, unconjugated (TMAB-05258) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

UniProt ID：

P26358

克隆号：

6Q563

基因名称：

DNMT1

货号：

T014|TMAB-05258