抗体 Anti-Cytokeratin 6 Polyclonal Antibody|TMAB-04959|TargetMol

中文名称：

抗体 Anti-Cytokeratin 6 Polyclonal Antibody

英文名称：

Anti-Cytokeratin 6 Polyclonal Antibody

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature -20°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

WB,FCM

反应性：

Human,Mouse (predicted:Rat,Pig,Cow,Horse,Rabbit,Sheep)

宿主：

Rabbit

靶点：

KRT6A

克隆性：

Polyclonal

免疫原：

KLH conjugated synthetic peptide derived from human Cytokeratin 6

亚型：

IgG

验证方法：

1. Sample: Tongue (Mouse) Lysate at 40 μg Primary: Anti-Cytokeratin 6 (TMAB-04959) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD 2. Blank control (black line): MCF-7. Primary Antibody (green line): Rabbit Anti-Cytokeratin 6 antibody (TMAB-04959) Dilution: 2 μg/Test; Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC Dilution: 0.5 μg/Test. Isotype control (orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

UniProt ID：

P02538

浓度：

1mg/ml

基因名称：

KRT6A

货号：

T014|TMAB-04959