抗体 Anti-EpCAM Antibody (9O49)|TMAB-05610|TargetMol

中文名称：

抗体 Anti-EpCAM Antibody (9O49)

英文名称：

Anti-EpCAM Antibody (9O49)

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature -20°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Recombinant Antibody

种属：

Human

应用：

WB,IHC-P,IHC-Fr,IF

反应性：

Human

宿主：

Rabbit

靶点：

EPCAM

克隆性：

Monoclonal

免疫原：

A synthesized peptide: human EpCAM

亚型：

IgG

验证方法：

1. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (EpCAM) Monoclonal Antibody, Unconjugated (TMAB-05610) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Human Thyroid tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EpCAM Monoclonal Antibody, Unconjugated (TMAB-05610) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EpCAM Monoclonal Antibody, Unconjugated (TMAB-05610) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EpCAM Monoclonal Antibody, Unconjugated (TMAB-05610) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EpCAM Monoclonal Antibody, Unconjugated (TMAB-05610) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EpCAM Monoclonal Antibody, Unconjugated (TMAB-05610) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EpCAM Monoclonal Antibody, Unconjugated (TMAB-05610) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EpCAM Monoclonal Antibody, Unconjugated (TMAB-05610) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 9. 25 μg total protein per lane of various lysates (see on figure) probed with EpCAM monoclonal antibody, unconjugated (TMAB-05610) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

UniProt ID：

P16422

克隆号：

9O49

浓度：

1mg/ml

基因名称：

EPCAM

货号：

T014|TMAB-05610