线粒体融合蛋白Mfn2抗体|TMAB-01132|TargetMol

中文名称：

抗体 Anti-MFN2 Polyclonal Antibody

英文名称：

Anti-MFN2 Polyclonal Antibody

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature Store at -20°C Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Human

应用：

WB,IHC-P,ICC/IF,ELISA

反应性：

Human,Mouse,Rat

宿主：

Rabbit

偶联物：

Unconjugated

靶点：

MFN2

克隆性：

Polyclonal

免疫原：

The antiserum was produced against synthesized peptide derived from human Mfn2.

亚型：

IgG

验证方法：

1. Tissue/cell: human kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Mitofusin-2 Polyclonal Antibody, Unconjugated (TMAB-01132) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated used at 1:200 dilution for 40 minutes at 37°C. 2. Tissue/cell: human breast cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Mitofusin 2 Polyclonal Antibody, Unconjugated (TMAB-01132) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Mitofusin 2) Polyclonal Antibody, Unconjugated (TMAB-01132) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Sample: Lane 1: Mouse Muscle tissue lysates Lane 2: Mouse Cerebrum tissue lysates Lane 3: Rat Muscle tissue lysates Lane 4: Rat Cerebrum tissue lysates Primary: Anti-Mitofusin 2 (TMAB-01132) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 83 kDa Observed band size: 77 kDa 5. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-Mitofusin 2 (TMAB-01132) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 83 kDa Observed band size: 68 kDa

UniProt ID：

O95140

浓度：

1 mg/mL

基因名称：

MFN2

货号：

T014|TMAB-01132