抗体 Anti-Cdkn1c Polyclonal Antibody|TMAB-04179|TargetMol

中文名称：

抗体 Anti-Cdkn1c Polyclonal Antibody

英文名称：

Anti-Cdkn1c Polyclonal Antibody

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature -20°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Polyclonal Antibody

种属：

Rat

应用：

WB,IHC-P,IHC-Fr,IF,FCM

反应性：

Human,Mouse,Rat (predicted:Cow,Sheep)

宿主：

Rabbit

靶点：

Cdkn1c

克隆性：

Polyclonal

免疫原：

KLH conjugated synthetic peptide derived from rat Cdkn1c

亚型：

IgG

验证方法：

1. Blank control: SH-SY5Y. Primary Antibody (green line): Rabbit Anti-p57 Kip2/Cdkn1c antibody (TMAB-04179) Dilution: 1 μg/Test; Secondary Antibody: Goat anti-rabbit IgG-FITC Dilution: 0.5 μg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. 2. Paraformaldehyde-fixed, paraffin embedded (Mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p57 Kip2) Polyclonal Antibody, Unconjugated (TMAB-04179) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p57 Kip2) Polyclonal Antibody, Unconjugated (TMAB-04179) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Blank control: Hela. Primary Antibody (green line): Rabbit Anti-p57 Kip2/Cdkn1c antibody (TMAB-04179) Dilution: 1 μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE Dilution: 1 μg /test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. 5. Sample: Lane 1: Rat Placenta tissue lysates Lane 2: Rat Cerebrum tissue lysates Lane 3: Human SH-SY5Y cell lysates Lane 4: Human HeLa cell lysates Lane 5: Human U-2 OS cell lysates Lane 6: Human Jurkat cell lysates Primary: Anti-Cdkn1c (TMAB-04179) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kDa Observed band size: 61 kDa

UniProt ID：

Q69DC0

浓度：

1mg/ml

基因名称：

Cdkn1c

货号：

T014|TMAB-04179